8FWF
Crystal structure of Apo form Fab235
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-06-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 120.755, 120.755, 86.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.590 - 1.940 |
| R-factor | 0.1722 |
| Rwork | 0.171 |
| R-free | 0.20570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.042 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.970 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Rmerge | 0.126 | 0.973 |
| Rmeas | 0.142 | |
| Rpim | 0.065 | 0.532 |
| Number of reflections | 46067 | 2312 |
| <I/σ(I)> | 15.5 | 1.52 |
| Completeness [%] | 96.7 | 98.9 |
| Redundancy | 4.4 | 4.1 |
| CC(1/2) | 0.985 | 0.505 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 2.0M Ammonium Sulphate, 0.1M HEPES:NaOH, 2%(v/v) PEG400 |
