8FWF

Crystal structure of Apo form Fab235

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97918
Spacegroup name	P 42 21 2
Unit cell lengths	120.755, 120.755, 86.940
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.590 - 1.940
R-factor	0.1722
Rwork	0.171
R-free	0.20570
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.042
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	HKL-3000
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.970
High resolution limit [Å]	1.940	1.940
Rmerge	0.126	0.973
Rmeas	0.142
Rpim	0.065	0.532
Number of reflections	46067	2312
<I/σ(I)>	15.5	1.52
Completeness [%]	96.7	98.9
Redundancy	4.4	4.1
CC(1/2)	0.985	0.505

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	289	2.0M Ammonium Sulphate, 0.1M HEPES:NaOH, 2%(v/v) PEG400