8FV2
Bromodomain of CBP liganded with CCS-1477
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2020-02-07 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.54178 |
Spacegroup name | P 41 |
Unit cell lengths | 79.453, 79.453, 97.127 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.440 - 1.870 |
R-factor | 0.1908 |
Rwork | 0.190 |
R-free | 0.22590 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.110 |
Data reduction software | HKL-2000 (719.2) |
Data scaling software | HKL-2000 (719.2) |
Phasing software | PHENIX (1.20.1-4487) |
Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 41.440 | 1.900 |
High resolution limit [Å] | 1.870 | 1.870 |
Rmeas | 0.155 | 0.966 |
Number of reflections | 49406 | 2111 |
<I/σ(I)> | 9.9 | 0.8 |
Completeness [%] | 99.1 | 84 |
Redundancy | 4 | 2.4 |
CC(1/2) | 0.985 | 0.535 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.2 | 293 | 0.056M Sodium phosphate monobasic monohydrate, 1.344M Potassium phosphate dibasic |