8FUB

Crystal structure of human Importin alpha 3 in complex with Hendra virus matrix protein NLS1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-07-25
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	49.060, 64.270, 158.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.780 - 2.750
R-factor	0.243
Rwork	0.241
R-free	0.28110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6bvz
RMSD bond length	0.003
RMSD bond angle	0.570
Data reduction software	MOSFLM (1.14_3260)
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.780	2.900
High resolution limit [Å]	2.750	2.750
Rmerge	0.181	1.032
Rpim		0.446
Number of reflections	13681	1966
<I/σ(I)>	7.8	2.3
Completeness [%]	99.9
Redundancy	11.3
CC(1/2)	0.995	0.887

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	0.1M sodium HEPES,0.72M sodium citrate, 1% dithiothreitol