8FSW
Crystal structure of hen egg white lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OXFORD DIFFRACTION ENHANCE ULTRA |
| Temperature [K] | 125 |
| Detector technology | CCD |
| Collection date | 2021-01-14 |
| Detector | OXFORD TITAN CCD |
| Wavelength(s) | 1.54056 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 77.357, 77.357, 37.164 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.050 - 1.600 |
| R-factor | 0.22421 |
| Rwork | 0.222 |
| R-free | 0.26518 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.676 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.050 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.277 | 5.957 |
| Rmeas | 0.314 | 7.201 |
| Rpim | 0.141 | 3.971 |
| Total number of observations | 72224 | 2258 |
| Number of reflections | 15248 | 788 |
| <I/σ(I)> | 7.5 | 0.6 |
| Completeness [%] | 99.1 | |
| Redundancy | 4.7 | 2.9 |
| CC(1/2) | 0.930 | 0.159 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 4.6 | 295 | 1.2 M NaCl, 100 mM Sodium Acetate, 50 mg/mL Lyzozyme, 30% Glycerol for cryoprotection |
