8FSO
Crystal structure of integrin beta-6 tail bound to the FERM-folded talin head domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-28 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.92011 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 66.777, 67.347, 115.007 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.090 - 2.330 |
| R-factor | 0.2098 |
| Rwork | 0.208 |
| R-free | 0.25230 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.894 |
| Data reduction software | Adxv |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.100 | 2.400 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Rmerge | 0.074 | 0.740 |
| Number of reflections | 22692 | 1620 |
| <I/σ(I)> | 14.4 | |
| Completeness [%] | 99.7 | |
| Redundancy | 3.5 | |
| CC(1/2) | 0.998 | 0.820 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 300 | PEG3350 20%, NaCl 0.1M, DTT 2mM |
