8FSL
Human Mesothelin bound to a neutralizing VH domain antibody
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-1 |
Synchrotron site | SSRL |
Beamline | BL12-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-07-31 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.98008 |
Spacegroup name | P 43 2 2 |
Unit cell lengths | 114.116, 114.116, 340.740 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 39.900 - 2.900 |
R-factor | 0.2391 |
Rwork | 0.238 |
R-free | 0.25390 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.339 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | CRANK2 |
Refinement software | PHENIX (1.19.2-4158_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 114.000 | 2.990 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.060 | 0.060 |
Rmeas | 0.384 | 0.360 |
Rpim | 0.055 | 0.470 |
Number of reflections | 51008 | 4354 |
<I/σ(I)> | 15.6 | 111 |
Completeness [%] | 99.0 | 99 |
Redundancy | 109 | 110 |
CC(1/2) | 0.990 | 0.210 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 286 | PEG3350 20% 0.15 M ammonium nitrate |