8FSL

Human Mesothelin bound to a neutralizing VH domain antibody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-1
Synchrotron site	SSRL
Beamline	BL12-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-31
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98008
Spacegroup name	P 43 2 2
Unit cell lengths	114.116, 114.116, 340.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.900 - 2.900
R-factor	0.2391
Rwork	0.238
R-free	0.25390
Structure solution method	SAD
RMSD bond length	0.011
RMSD bond angle	1.339
Data reduction software	XDS
Data scaling software	XDS
Phasing software	CRANK2
Refinement software	PHENIX (1.19.2-4158_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	114.000	2.990
High resolution limit [Å]	2.900	2.900
Rmerge	0.060	0.060
Rmeas	0.384	0.360
Rpim	0.055	0.470
Number of reflections	51008	4354
<I/σ(I)>	15.6	111
Completeness [%]	99.0	99
Redundancy	109	110
CC(1/2)	0.990	0.210

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		286	PEG3350 20% 0.15 M ammonium nitrate