8FSK
LSD1-CoREST in complex with T18, short soaking
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-06-22 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9677 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 119.391, 179.911, 235.331 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.700 - 3.130 |
| R-factor | 0.1996 |
| Rwork | 0.199 |
| R-free | 0.22260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.356 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 106.470 | 3.240 |
| High resolution limit [Å] | 3.120 | 3.120 |
| Rmerge | 0.158 | 4.539 |
| Rmeas | 0.164 | 4.713 |
| Rpim | 0.044 | 1.252 |
| Total number of observations | 619486 | 58842 |
| Number of reflections | 44804 | 4305 |
| <I/σ(I)> | 13.9 | 0.6 |
| Completeness [%] | 98.5 | |
| Redundancy | 13.8 | 13.7 |
| CC(1/2) | 0.999 | 0.299 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1.2 Sodium/Potassium Tartrate, 100 mM ADA pH 6.5 |
