8FRT

X-ray crystal structure of the N-terminal region from HCMV US11 binding to HLA-A*02:01

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.954
Spacegroup name	P 1 21 1
Unit cell lengths	52.386, 80.503, 56.082
Unit cell angles	90.00, 113.49, 90.00

Refinement procedure

Resolution	48.040 - 1.800
Rwork	0.206
R-free	0.23890
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (v1.20.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.040	1.840
High resolution limit [Å]	1.800	1.800
Number of reflections	39611	2325
<I/σ(I)>	6.8
Completeness [%]	100.0
Redundancy	3.5
CC(1/2)	0.996	0.704

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	30% (w/v) PEG3350, 0.1 M DL-malic acid pH 7.0, 83 mM cesium chloride