8FRK

Structure of nsp14 N7-MethylTransferase domain fused with TELSAM bound to SGC8158

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.920105
Spacegroup name	P 65
Unit cell lengths	109.174, 109.174, 48.659
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.270 - 1.610
R-factor	0.2189
Rwork	0.217
R-free	0.25110
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.775
Data reduction software	XDS (Jan 10, 2022 (BUILT 20220110))
Data scaling software	Aimless (0.7.7)
Phasing software	PHASER
Refinement software	PHENIX (1.20rc3_4406)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	94.547	1.859
High resolution limit [Å]	1.606	1.606
Rmerge	0.062	0.851
Rmeas	0.073
Rpim	0.038	0.534
Number of reflections	23244	1163
<I/σ(I)>	9.3
Completeness [%]	94.3	68.6
Redundancy	7
CC(1/2)	0.999	0.676

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	293.15	11-17% Reagent Alcohol, 0.1-0.3M Lithium Sulfate and 0.1M Sodium Citrate pH 5.5