8FPW

Crystal structure of tumor related RhoA mutant A161V in complex with GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 31-ID
Synchrotron site	APS
Beamline	31-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-03-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9793
Spacegroup name	P 1 21 1
Unit cell lengths	32.010, 86.844, 34.337
Unit cell angles	90.00, 93.75, 90.00

Refinement procedure

Resolution	26.900 - 1.400
R-factor	0.1798
Rwork	0.178
R-free	0.20790
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.993
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.22)
Phasing software	PHASER
Refinement software	PHENIX (1.19.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.900	1.480
High resolution limit [Å]	1.400	1.400
Rmerge	0.108	0.379
Rmeas	0.143	0.554
Rpim	0.074	0.312
Number of reflections	35470	4578
<I/σ(I)>	5.2	1.8
Completeness [%]	96.5	86
Redundancy	3.6	2.9
CC(1/2)	0.985	0.738

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	20-24% PEG8K, 15% Dioxane, 0.1M Tris pH8.0