8FPQ

PCSK9 in complex with an inhibitor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-11-14
Detector	DECTRIS EIGER R 1M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	62.414, 70.369, 150.829
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.671 - 2.503
R-factor	0.1907
Rwork	0.188
R-free	0.23440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.636
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	FFT
Refinement software	PHENIX (1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	150.000	2.512
High resolution limit [Å]	2.500	2.500
Rmerge	0.170	1.299
Number of reflections	140840	14084
<I/σ(I)>	11.7
Completeness [%]	99.1
Redundancy	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	20.0% Peg-6000, 0.1M Tris pH8.0