8FP8

Crystal structure of hen egg white lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	OXFORD DIFFRACTION ENHANCE ULTRA
Temperature [K]	125
Detector technology	CCD
Collection date	2020-08-14
Detector	OXFORD TITAN CCD
Wavelength(s)	1.54056
Spacegroup name	P 43 21 2
Unit cell lengths	78.380, 78.380, 36.934
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	14.080 - 1.600
R-factor	0.19516
Rwork	0.194
R-free	0.21890
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.751
Data reduction software	HKL-2000
Data scaling software	Aimless
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	14.070	1.630
High resolution limit [Å]	1.600	1.600
Rmerge	0.050	0.352
Rmeas	0.056	0.428
Rpim	0.024	0.239
Total number of observations	73186	2263
Number of reflections	15627	762
<I/σ(I)>	16.2	2.8
Completeness [%]	99.5
Redundancy	4.7	3
CC(1/2)	0.998	0.863

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	4.6	295	1.2 M NaCl, 100 mM Sodium Acetate, 50 mg/mL Lyzozyme, 30% Glycerol for cryoprotection