8FP5
CDK2 liganded with ATP and Mg2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2022-11-20 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 53.670, 71.710, 72.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.600 - 1.700 |
| R-factor | 0.196 |
| Rwork | 0.194 |
| R-free | 0.23040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b39 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.184 |
| Data reduction software | XDS (Jan 10, 2022) |
| Data scaling software | XSCALE (Jan 10, 2022) |
| Phasing software | PHENIX (1.20.1-4487) |
| Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.600 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmeas | 0.035 | 1.040 |
| Number of reflections | 31044 | 2197 |
| <I/σ(I)> | 23.7 | 1.3 |
| Completeness [%] | 99.3 | 96.5 |
| Redundancy | 3.6 | 2.7 |
| CC(1/2) | 1.000 | 0.550 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 10% PEG 3350, 10% ethylene glycol, 50mM HEPES, pH 7.5 |
