8FNB
Crystal structure of the C-terminal Fg domain of human TNC with the mutations Y2140H and S2164H
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-02 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.045, 101.561, 56.667 |
| Unit cell angles | 90.00, 122.35, 90.00 |
Refinement procedure
| Resolution | 34.830 - 1.800 |
| R-factor | 0.1528 |
| Rwork | 0.152 |
| R-free | 0.17380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2j3f |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.872 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.068 | 0.250 |
| Rpim | 0.028 | 0.104 |
| Number of reflections | 46455 | 4286 |
| <I/σ(I)> | 20.8 | 4.8 |
| Completeness [%] | 98.9 | |
| Redundancy | 6.9 | |
| CC(1/2) | 0.992 | 0.987 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 200 mM lithium sulfate, 25% PEG1500, 50 mM Tris, pH 8.0 |
