8FNB

Crystal structure of the C-terminal Fg domain of human TNC with the mutations Y2140H and S2164H

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2014-08-02
Detector	RAYONIX MX300-HS
Wavelength(s)	1.00
Spacegroup name	C 1 2 1
Unit cell lengths	106.045, 101.561, 56.667
Unit cell angles	90.00, 122.35, 90.00

Refinement procedure

Resolution	34.830 - 1.800
R-factor	0.1528
Rwork	0.152
R-free	0.17380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2j3f
RMSD bond length	0.009
RMSD bond angle	0.872
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.068	0.250
Rpim	0.028	0.104
Number of reflections	46455	4286
<I/σ(I)>	20.8	4.8
Completeness [%]	98.9
Redundancy	6.9
CC(1/2)	0.992	0.987

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	200 mM lithium sulfate, 25% PEG1500, 50 mM Tris, pH 8.0