8FMM

Complex structure of wild type Troponin complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-13
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97973
Spacegroup name	P 1 21 1
Unit cell lengths	42.071, 168.219, 69.683
Unit cell angles	90.00, 101.40, 90.00

Refinement procedure

Resolution	41.241 - 3.112
R-factor	0.2313
Rwork	0.230
R-free	0.26380
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MOLREP
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.260
High resolution limit [Å]	3.110	3.110
Rmerge	0.200	1.800
Number of reflections	16901	1695
<I/σ(I)>	12.6	1.94
Completeness [%]	99.1
Redundancy	7.7
CC(1/2)		0.483

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.2M sodium acetate, 20% PEG 3350