8FMH
Structure of CBASS Cap5 from Pseudomonas syringae as an activated tetramer with the cyclic dinucleotide 3'2'-c-dGAMP ligand (2 tetramers in the AU)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-21 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.920105 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.970, 295.678, 84.052 |
| Unit cell angles | 90.00, 113.79, 90.00 |
Refinement procedure
| Resolution | 38.450 - 1.870 |
| R-factor | 0.212 |
| Rwork | 0.210 |
| R-free | 0.25330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8fmf |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.615 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 147.839 | 2.112 |
| High resolution limit [Å] | 1.870 | 1.871 |
| Rmerge | 0.125 | 0.753 |
| Number of reflections | 151543 | 7577 |
| <I/σ(I)> | 6.9 | |
| Completeness [%] | 93.6 | |
| Redundancy | 4 | |
| CC(1/2) | 0.994 | 0.644 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Bis-Tris, pH 5.5-6.0, 0.2 M magnesium chloride, 19-20% PEG3350 |
