8FK6

Crystal Structure of the Tick Evasin EVA-AAM1001(Y44A) Complexed to Human Chemokine CCL7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953
Spacegroup name	P 41 21 2
Unit cell lengths	72.301, 72.301, 67.275
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.700 - 1.740
R-factor	0.207
Rwork	0.206
R-free	0.22320
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.632
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.250	1.770
High resolution limit [Å]	1.740	1.740
Number of reflections	18937	1025
<I/σ(I)>	39.4
Completeness [%]	99.9
Redundancy	13.9
CC(1/2)	0.999	0.985

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	2 M Ammonium sulfate