8FJE

The five-repeat design E8

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-21
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 2 21 21
Unit cell lengths	47.859, 65.763, 87.434
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.560 - 3.000
R-factor	0.2706
Rwork	0.267
R-free	0.29930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.437
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (dev_4761)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	52.560	3.300
High resolution limit [Å]	3.000	3.000
Rmerge	0.203	1.240
Rpim	0.060	0.346
Number of reflections	5818	1428
<I/σ(I)>	7.95	2.48
Completeness [%]	98.8	99.72
Redundancy	12.7	13.6
CC(1/2)	0.993	0.903

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2M DL-Glutamic acid monohydrate, 0.2M, DL-Alanine, 0.2M Glycine, 0.2M DL-Lysine, 1.0M imidazole, MES monohydrate (acid), 37.5 % v/v of (25% v/v MPD; 25% PEG 1000; 25% w/v PEG 3350)