8FJ9
Structure of the catalytic domain of Streptococcus mutans GtfB in tetragonal space group P4322
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-06-11 |
| Detector | DECTRIS EIGER2 S 16M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 150.130, 150.130, 302.783 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 75.700 - 2.500 |
| R-factor | 0.21363 |
| Rwork | 0.212 |
| R-free | 0.24086 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.299 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 86.920 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.314 | |
| Rmeas | 0.322 | |
| Rpim | 0.072 | 0.882 |
| Total number of observations | 2410102 | |
| Number of reflections | 119867 | 5852 |
| <I/σ(I)> | 9.3 | |
| Completeness [%] | 100.0 | 99.8 |
| Redundancy | 20.1 | 21 |
| CC(1/2) | 0.996 | 0.439 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 2 M ammonium sulfate, 0.1 M Bis-Tris |
