8FIH

Multi-state design of two-state switchable hinge proteins

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00
Spacegroup name	P 21 21 2
Unit cell lengths	66.018, 119.757, 38.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.350 - 2.200
R-factor	0.1946
Rwork	0.191
R-free	0.24840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.559
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.350	2.300
High resolution limit [Å]	2.200	2.200
Rmerge	0.030	1.161
Number of reflections	15520	1653
<I/σ(I)>	9.89	2.16
Completeness [%]	95.3
Redundancy	1.4	1
CC(1/2)	0.999	0.852

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	293	0.2 M Lithium sulfate, 0.1 M Sodium Phosphate-citrate pH 4.2, 20% PEG 1000