8FHU
Structure of Pyruvate dehydrogenase phosphatase regulatory subunit epitope presented by H2-Dd
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-23 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979180 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.415, 104.564, 169.993 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.819 - 1.801 |
| Rwork | 0.208 |
| R-free | 0.24880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.237 |
| Data reduction software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0403) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.930 |
| High resolution limit [Å] | 1.801 | 1.890 |
| Rmerge | 0.177 | |
| Rmeas | 0.185 | |
| Rpim | 0.052 | |
| Number of reflections | 81134 | 3638 |
| <I/σ(I)> | 13.2 | |
| Completeness [%] | 99.0 | 82.6 |
| Redundancy | 13.2 | 11.7 |
| CC(1/2) | 0.998 | 0.261 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298.15 | 0.2M Potassium thiocyanate and 20% PEG 3350 |
