8FHJ

Crystal structure of a FAD monooxygenease from Methylocystis sp. Strain SB2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-04-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9793
Spacegroup name	C 1 2 1
Unit cell lengths	148.347, 96.765, 135.206
Unit cell angles	90.00, 116.41, 90.00

Refinement procedure

Resolution	59.950 - 2.610
R-factor	0.2062
Rwork	0.204
R-free	0.25380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ihg
RMSD bond length	0.008
RMSD bond angle	0.994
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.18.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	121.100	2.750
High resolution limit [Å]	2.610	2.610
Rmerge	0.126	1.692
Rmeas	0.149	1.986
Rpim	0.079	1.033
Total number of observations	184364	27645
Number of reflections	52358	7601
<I/σ(I)>	6.4	0.8
Completeness [%]	99.8
Redundancy	3.5	3.6
CC(1/2)	0.996	0.461

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	20% PEG3350, 0.1 M Bis-Tris propane pH 6.5, 0.2 M Sodium bromide