8FHE

Crystal structure of PPARgamma ligand-binding domain in complex with N-CoR peptide and GW9662

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 41 21 2
Unit cell lengths	61.721, 61.721, 163.918
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.140 - 1.800
R-factor	0.1987
Rwork	0.197
R-free	0.22860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.864
Data reduction software	DIALS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.140	1.864
High resolution limit [Å]	1.800	1.800
Rmerge	0.013	0.157
Rmeas	0.019
Rpim	0.013	0.157
Number of reflections	30317	2980
<I/σ(I)>	28
Completeness [%]	99.9
Redundancy	2	2
CC(1/2)	1.000	0.941

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.2M Ammonium sulfate, 0.1M MES, pH 6.5, 30% w/v, PEG 8000