8FHA
Intramolecular ester bond-containing repeat domain 1 from Suipraoptans intestinalis adhesin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-07-07 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.95372 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.051, 104.712, 52.587 |
| Unit cell angles | 90.00, 91.81, 90.00 |
Refinement procedure
| Resolution | 48.027 - 1.490 |
| Rwork | 0.166 |
| R-free | 0.21210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.309 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.090 | 1.520 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Number of reflections | 81829 | 3553 |
| <I/σ(I)> | 10.5 | |
| Completeness [%] | 96.6 | 84.8 |
| Redundancy | 5.9 | 5.4 |
| CC(1/2) | 0.999 | 0.350 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.1 M HEPES, 70% MPD |
