8FFT

Structure of GntC, a PLP-dependent enzyme catalyzing L-enduracididine biosynthesis from (S)-4-hydroxy-L-arginine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-09-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.99992
Spacegroup name	P 1 21 1
Unit cell lengths	60.855, 158.159, 73.231
Unit cell angles	90.00, 90.01, 90.00

Refinement procedure

Resolution	73.230 - 2.100
R-factor	0.2211
Rwork	0.220
R-free	0.24170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3op7
Data reduction software	AutoProcess
Data scaling software	AutoProcess
Phasing software	AutoProcess
Refinement software	PHENIX (1.18_3845)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	73.230	2.106
High resolution limit [Å]	2.100	2.100
Number of reflections	75093	778
<I/σ(I)>	10.9
Completeness [%]	93.1
Redundancy	5.2
CC(1/2)		0.573

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		283	19% (w/v) PEG 3350, 0.50 M MgCl2, 0.10 M Tris pH 8.5, and 1 mM PLP