8FEQ

16mer self-complementary duplex RNA with two separated s(2)U:s(2)U pairs

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 2.0.1
Synchrotron site	ALS
Beamline	2.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-14
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.037690
Spacegroup name	H 3 2
Unit cell lengths	41.261, 41.261, 124.239
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.993 - 1.500
Rwork	0.177
R-free	0.21030
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.021
RMSD bond angle	2.577
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.000	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.077	0.235
Rmeas	0.082	0.249
Rpim	0.029	0.081
Number of reflections	6853	352
<I/σ(I)>	14.2	2.3
Completeness [%]	100.0	100
Redundancy	8.7	9
CC(1/2)	0.998	0.973

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	0.075 M Sodium chloride, 0.002 M Calcium chloride dihydrate, 0.05 M Sodium cacodylate trihydrate pH 6.0, 30% w/v 1,6 Hexanediol, 0.0005 M Spermine