8FEO

16mer self-complementary duplex RNA with two separated native U:U pairs

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033175
Spacegroup name	H 3 2
Unit cell lengths	41.503, 41.503, 125.299
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	17.796 - 1.520
Rwork	0.198
R-free	0.23930
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	2.559
Data reduction software	xia2
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	17.800	1.550
High resolution limit [Å]	1.520	1.520
Rmerge	0.075	0.234
Rmeas	0.081	0.253
Rpim	0.029	0.093
Number of reflections	6587	322
<I/σ(I)>	36.4	3
Completeness [%]	98.3	99.1
Redundancy	8.4	7
CC(1/2)	0.998	0.973

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.08 M Strontium chloride hexahydrate, 0.02 M Magnesium chloride hexahydrate, 0.04 M Sodium cacodylate trihydrate pH 7.0, 20% v/v (+/-)-2-Methyl-2,4-pentanediol, 0.012 M Spermine tetrahydrochloride