8FDV
LSD1-CoREST in complex N-formyl FAD and SNAG peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-22 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.96546 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 119.298, 180.388, 232.884 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.920 - 2.950 |
| R-factor | 0.2176 |
| Rwork | 0.216 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.549 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.920 | 3.040 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.109 | 1.619 |
| Rmeas | 0.124 | 1.865 |
| Rpim | 0.058 | 0.905 |
| Total number of observations | 222491 | 18249 |
| Number of reflections | 52843 | 4534 |
| <I/σ(I)> | 8.2 | 0.8 |
| Completeness [%] | 99.5 | |
| Redundancy | 4.2 | 4 |
| CC(1/2) | 0.996 | 0.332 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 1.2 Na/K Tartrate, 100 mM ADA pH 6.5 |
