8FDS
Ankyrin domain of SKD3 isoform 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 60.440, 60.440, 180.313 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 52.340 - 1.650 |
| R-factor | 0.2002 |
| Rwork | 0.199 |
| R-free | 0.22070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8deh |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.863 |
| Data reduction software | DIALS (3.6.2) |
| Data scaling software | DIALS (3.6.2) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (1.20.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.340 | 1.680 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.101 | 0.532 |
| Rmeas | 0.104 | |
| Rpim | 0.024 | 0.125 |
| Number of reflections | 24410 | 1202 |
| <I/σ(I)> | 9 | 0.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 18.3 | 19.1 |
| CC(1/2) | 0.999 | 0.876 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 287 | 4 M potassium formate, 0.1 M Bis-Tris propane, pH 9.0, 2% w/v PEG2000 MME |
