8FCZ

Crystal structure of ground-state rhodopsin in complex with a nanobody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 31 2 1
Unit cell lengths	119.277, 119.277, 227.115
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.760 - 3.700
R-factor	0.2834
Rwork	0.281
R-free	0.33180
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.718
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.920
High resolution limit [Å]	3.700	3.700
Rmerge	0.094	2.831
Number of reflections	20570	3236
<I/σ(I)>	10.22	0.71
Completeness [%]	99.8
Redundancy	9.8
CC(1/2)	0.999	0.378

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277.15	30% PEG 600 0.1 M Tricine pH 8 1.2 mM F3215-0002 2% IPTG 50 mM phenol 5 mM EDTA 5 mM beta mercaptoethanol 50 mM Cyglu-4