8FC1

Crystal structure of the Thermus thermophilus 70S ribosome in complex with protein Y, hygromycin A, and erythromycin at 2.50A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-07-18
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	208.570, 448.580, 617.470
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	151.650 - 2.500
R-factor	0.2078
Rwork	0.206
R-free	0.24630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6xhx
RMSD bond length	0.004
RMSD bond angle	0.883
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	151.650	151.650	2.560
High resolution limit [Å]	2.500	11.180	2.500
Rmerge	0.169	0.047	2.402
Rmeas	0.183	0.051	2.586
Total number of observations	13707917
Number of reflections	1965632	23144	144933
<I/σ(I)>	9.13	34.06	0.86
Completeness [%]	99.9	99.8	100
Redundancy	6.974	6.989	7.207
CC(1/2)	0.995	0.995	0.203

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME