8FBN

Improving the secretion of designed protein assemblies through negative design of cryptic transmembrane domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2021-06-10
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9791
Spacegroup name	P 1 21 1
Unit cell lengths	71.997, 170.813, 72.148
Unit cell angles	90.00, 117.83, 90.00

Refinement procedure

Resolution	42.700 - 3.040
R-factor	0.2587
Rwork	0.255
R-free	0.30020
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.371
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.700	3.110
High resolution limit [Å]	3.040	3.040
Rmerge	0.151	1.631
Number of reflections	29424	4770
<I/σ(I)>	4.8	0.8
Completeness [%]	99.2	99.9
Redundancy	3.7	3.8
CC(1/2)	0.988	0.293

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.1 M Tris, pH 7.0, 20% w/v PEG2000 MME