8FBJ

Improving the secretion of designed protein assemblies through negative design of cryptic transmembrane domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-20
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.97918
Spacegroup name	H 3
Unit cell lengths	89.917, 89.917, 124.897
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.630 - 3.250
R-factor	0.2419
Rwork	0.240
R-free	0.28490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.336
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.990	3.510
High resolution limit [Å]	3.250	3.250
Rmerge	0.119	0.261
Number of reflections	5943	11095
<I/σ(I)>	10.2	5.5
Completeness [%]	100.0	100
Redundancy	8.6	8.6
CC(1/2)	0.998	0.975

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	0.2 M magnesium chloride, 0.1 M Tris, pH 7.0, 10% w/v PEG8000