8FAX

Fab 1249A8-MERS Stem Helix Complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	113
Detector technology	PIXEL
Collection date	2022-03-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	52.714, 56.308, 76.124
Unit cell angles	90.00, 91.19, 90.00

Refinement procedure

Resolution	43.760 - 2.100
R-factor	0.1853
Rwork	0.182
R-free	0.23970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.903
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.300	2.130
High resolution limit [Å]	2.100	2.100
Rmerge	0.119	0.436
Number of reflections	26255	5954
<I/σ(I)>	5
Completeness [%]	93.9
Redundancy	3
CC(1/2)	0.967

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	290	0.2 M magnesium chloride, 0.1 M Tris, pH 8.5, 20% PEG8000