8FA5
Crystal structure of Xanthomonas campestris GH35 beta-galactosidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-25 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.865, 84.768, 99.811 |
| Unit cell angles | 90.00, 96.61, 90.00 |
Refinement procedure
| Resolution | 42.793 - 1.700 |
| R-factor | 0.1712 |
| Rwork | 0.170 |
| R-free | 0.20260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.227 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (v1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.800 | 1.750 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 283540 | 27563 |
| <I/σ(I)> | 13.7 | |
| Completeness [%] | 97.6 | |
| Redundancy | 4.3 | |
| CC(1/2) | 0.990 | 0.610 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.2M lithium chloride, 20% PEG 3350 |
