8F9O

Dog sialic acid esterase (SIAE)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-15
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0
Spacegroup name	I 2 2 2
Unit cell lengths	111.702, 112.480, 122.243
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.850 - 1.250
R-factor	0.1027
Rwork	0.102
R-free	0.12010
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.408
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.290
High resolution limit [Å]	1.250	1.250
Rmeas	0.096
Number of reflections	413696	33962
<I/σ(I)>	27.1
Completeness [%]	98.0
Redundancy	12.1
CC(1/2)		0.480

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.01 M tri-sodium citrate, 16% w/v PEG6000