8F9E

Crystal structure of Ky15.2 Fab in complex with circumsporozoite protein KQPA peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033192
Spacegroup name	P 21 21 21
Unit cell lengths	78.167, 85.209, 133.475
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.440 - 2.950
R-factor	0.2177
Rwork	0.216
R-free	0.24130
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.571
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.19_4080)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.000
High resolution limit [Å]	2.950	2.950
Number of reflections	19407	934
<I/σ(I)>	7.1
Completeness [%]	99.8
Redundancy	13.3
CC(1/2)	0.992	0.795

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1 M HEPES pH 7.5, 25% (w/v) PEG3350, 0.2 M sodium chloride