8F8Z
PHF2 (PHD+JMJ) in Complex with H3 Histone N-Terminal Peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-10-06 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 73.854, 83.756, 104.128 |
Unit cell angles | 90.00, 103.67, 90.00 |
Refinement procedure
Resolution | 54.500 - 3.300 |
R-factor | 0.2154 |
Rwork | 0.213 |
R-free | 0.25600 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.421 |
Data reduction software | DIALS |
Data scaling software | DIALS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 54.500 | 3.400 |
High resolution limit [Å] | 3.300 | 3.300 |
Number of reflections | 36233 | 3642 |
<I/σ(I)> | 3.4 | 0.9 |
Completeness [%] | 98.6 | 91.5 |
Redundancy | 7.8 | |
CC(1/2) | 0.929 | 0.434 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.3 | 292 | 2.5M Ammonium Sulfate 90mM Bis-Tris Propane, pH 6.3 4% Pentaerythritol ethoxylate (3/4 EO/OH) |