8F73

Crystal structure of Pseudomonas aeruginosa UDP-glucose phosphorylase in complex with UDP-glucose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.953722
Spacegroup name	P 1 21 1
Unit cell lengths	84.715, 114.847, 155.761
Unit cell angles	90.00, 97.39, 90.00

Refinement procedure

Resolution	46.980 - 2.900
R-factor	0.1968
Rwork	0.195
R-free	0.23570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3juk
RMSD bond length	0.007
RMSD bond angle	0.997
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.520	2.970
High resolution limit [Å]	2.900	2.900
Rmerge	0.109	0.860
Rpim		0.580
Number of reflections	65768	4608
<I/σ(I)>	9.3	1.8
Completeness [%]	99.7
Redundancy	3.9
CC(1/2)	0.960	1.000

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M sodium acetate trihydrate pH 4.6 2.0 M sodium formate