8F6L
anti-BTLA monoclonal antibody h25F7 in complex with BTLA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-11-09 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.961, 68.600, 187.321 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.850 |
| R-factor | 0.20016 |
| Rwork | 0.198 |
| R-free | 0.24006 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2aw2 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.349 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (v5.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.000 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.083 | 0.672 |
| Number of reflections | 54860 | 7911 |
| <I/σ(I)> | 15.7 | 2.8 |
| Completeness [%] | 99.8 | 99.8 |
| Redundancy | 7.2 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 281 | 18% PEG 5000 MME + 200mM Magnesium Formate dihydrate + 100mM Sodium Acetate pH 4.6 |
