8F6L
anti-BTLA monoclonal antibody h25F7 in complex with BTLA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-09 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97931 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.961, 68.600, 187.321 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 1.850 |
R-factor | 0.20016 |
Rwork | 0.198 |
R-free | 0.24006 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2aw2 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.349 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (v5.8) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.000 | 1.950 |
High resolution limit [Å] | 1.850 | 1.850 |
Rmerge | 0.083 | 0.672 |
Number of reflections | 54860 | 7911 |
<I/σ(I)> | 15.7 | 2.8 |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 7.2 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 281 | 18% PEG 5000 MME + 200mM Magnesium Formate dihydrate + 100mM Sodium Acetate pH 4.6 |