8F6D
Crystal structure of the CNNM2 CBS-pair domain in complex with ARL15
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-04-14 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 |
Unit cell lengths | 66.018, 73.323, 79.327 |
Unit cell angles | 94.35, 95.50, 115.33 |
Refinement procedure
Resolution | 29.530 - 3.200 |
R-factor | 0.2655 |
Rwork | 0.264 |
R-free | 0.29270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4iy0 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.791 |
Data reduction software | DIALS |
Data scaling software | DIALS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.260 |
High resolution limit [Å] | 3.200 | 3.200 |
Number of reflections | 20526 | 1986 |
<I/σ(I)> | 10.4 | 0.6 |
Completeness [%] | 94.2 | 93.1 |
Redundancy | 3.7 | 3.8 |
CC(1/2) | 0.998 | 0.624 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 294 | 0.2 M sodium chloride, 0.1 M Tris pH 8.5, 25% (w/v) PEG3350, 0.5 mM GppNp |