8F69
Crystal structure of murine PolG2 dimer bound to DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2017-12-02 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 88.027, 88.027, 343.170 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.630 - 2.200 |
| R-factor | 0.1949 |
| Rwork | 0.194 |
| R-free | 0.22830 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g5i |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.633 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.630 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmeas | 0.127 | 1.490 |
| Number of reflections | 69448 | 6754 |
| <I/σ(I)> | 20.59 | 1.1 |
| Completeness [%] | 99.7 | 99.16 |
| Redundancy | 8.9 | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 20% MPD 0.1 M MES pH 6.5 |
