8F16
Structure of the STUB1 TPR domain in complex with H203, an all-D Helicon Polypeptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-12-19 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.18057 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.560, 66.880, 103.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.410 - 1.560 |
| R-factor | 0.197 |
| Rwork | 0.195 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q49 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.410 | 1.590 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Rmerge | 0.030 | 0.676 |
| Number of reflections | 50341 | |
| <I/σ(I)> | 22.4 | 2.2 |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 6.3 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M BICINE pH 8.5, 15% w/v PEG 1,500 |
