8F13
Structure of the MDM2 P53 binding domain in complex with H103, an all-D Helicon Polypeptide, alternative C-terminus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-03-31 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.03319 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 60.286, 60.286, 84.897 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.450 - 1.400 |
| Rwork | 0.174 |
| R-free | 0.20780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3g03 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2-3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.450 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.083 | 1.268 |
| Number of reflections | 31020 | 1334 |
| <I/σ(I)> | 23 | 2.3 |
| Completeness [%] | 98.4 | 86 |
| Redundancy | 20.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.2 M Potassium sodium tartrate, 0.1 M tri-Sodium citrate pH 5.6, 2.0 M Ammonium sulfate |
