8F0Z

Structure of the MDM2 P53 binding domain in complex with H101, an all-D Helicon Polypeptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-31
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.03319
Spacegroup name	P 21 21 2
Unit cell lengths	40.871, 81.357, 34.975
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.870 - 1.610
Rwork	0.221
R-free	0.24230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3g03
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.18.2-3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.870	1.640
High resolution limit [Å]	1.610	1.610
Rmerge	0.067
Number of reflections	15696	774
<I/σ(I)>	19.3	2.1
Completeness [%]	99.6	100
Redundancy	12.8	12.9
CC(1/2)	0.998	0.774

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	3.5 M Sodium Formate pH 7.0