8F0W

Tudor Domain of Tumor suppressor p53BP1 with MFP-5956

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	CCD
Collection date	2018-02-26
Detector	RIGAKU SATURN A200
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	48.479, 77.027, 87.208
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.010 - 1.520
R-factor	0.2122
Rwork	0.212
R-free	0.23440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2g3r
RMSD bond length	0.010
RMSD bond angle	1.429
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER (2.8.2)
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.010	50.000	1.550
High resolution limit [Å]	1.520	4.130	1.520
Rmerge	0.036	0.027	0.444
Rmeas	0.039	0.029	0.489
Rpim	0.014	0.010	0.198
Number of reflections	48449	2720	1974
<I/σ(I)>	15.7
Completeness [%]	94.7	97.7	79.1
Redundancy	7.5	7.1	5.5
CC(1/2)		0.999	0.831

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	2M ammonium sulfate, 0.1 M HEPES pH 7.5, 2% PEG 400