8EZW
Structure of Apo ZrgA deletion 124-184 from Vibrio cholerae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-10-13 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.00 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 81.055, 65.444, 88.735 |
Unit cell angles | 90.00, 98.77, 90.00 |
Refinement procedure
Resolution | 45.880 - 2.000 |
R-factor | 0.19425 |
Rwork | 0.192 |
R-free | 0.23630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7ahw |
RMSD bond length | 0.016 |
RMSD bond angle | 1.847 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (7.1.018) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.180 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.056 | 0.706 |
Rmeas | 0.065 | 0.820 |
Rpim | 0.033 | 0.410 |
Total number of observations | 236374 | 16684 |
Number of reflections | 62935 | 4390 |
<I/σ(I)> | 14.1 | 2.1 |
Completeness [%] | 99.0 | |
Redundancy | 3.8 | 3.8 |
CC(1/2) | 0.999 | 0.834 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | BATCH MODE | 5.5 | 296 | Protein at 10 mg/mL was combined at a 1:1 ratio with precipitant solution containing 0.2 M NaCl, 0.1 M bis-tris propane pH 5.5, 28% PEG 3350 |