8EZT

Crystal structure of HipB(Lp) from Legionella pneumophila

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2019-04-29
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 1 2 1
Unit cell lengths	59.444, 33.488, 74.161
Unit cell angles	90.00, 102.73, 90.00

Refinement procedure

Resolution	22.020 - 2.060
R-factor	0.2996
Rwork	0.297
R-free	0.34310
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wiu
RMSD bond length	0.004
RMSD bond angle	0.520
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.20_4459)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.000	2.100
High resolution limit [Å]	2.060	2.060
Rmerge	1.046	0.450
Rpim	0.054	0.271
Number of reflections	18042	888
<I/σ(I)>	15.6	2
Completeness [%]	99.8
Redundancy	3.9
CC(1/2)		0.911

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	0.2 M ammonium sulfate, 25% PEG 4K, 0.1 M sodium acetate pH 4.6. cryo paratone