8EYU

Structure of Beetroot dimer bound to DFAME

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.977
Spacegroup name	P 1 21 1
Unit cell lengths	29.937, 50.742, 95.555
Unit cell angles	90.00, 94.08, 90.00

Refinement procedure

Resolution	47.660 - 1.950
R-factor	0.1809
Rwork	0.178
R-free	0.20400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5bjo
RMSD bond length	0.004
RMSD bond angle	0.818
Data reduction software	xia2
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.660	2.020
High resolution limit [Å]	1.950	1.950
Rmerge	0.056
Rmeas	0.061
Number of reflections	21035	2095
<I/σ(I)>	16.32
Completeness [%]	99.8
Redundancy	6.5
CC(1/2)		0.985

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	294.15	2 M Ammonium sulfate, 5% 2-propanol