8EY9
Structure of Arabidopsis fatty acid amide hydrolase mutant S305A in complex with 9-hydroxy-10,12-octadecadienoyl-ethanolamide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL14-1 |
| Synchrotron site | SSRL |
| Beamline | BL14-1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2019-07-23 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 1.078090 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 222.906, 82.908, 271.784 |
| Unit cell angles | 90.00, 109.13, 90.00 |
Refinement procedure
| Resolution | 39.790 - 3.590 |
| R-factor | 0.2696 |
| Rwork | 0.269 |
| R-free | 0.30460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8eww |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.790 | 3.660 |
| High resolution limit [Å] | 3.590 | 3.590 |
| Rmerge | 0.163 | 0.724 |
| Number of reflections | 108547 | 4896 |
| <I/σ(I)> | 4.2 | 1.5 |
| Completeness [%] | 98.5 | 91.3 |
| Redundancy | 3.5 | 3.4 |
| CC(1/2) | 0.995 | 0.837 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1 M MES, PH 6.0, 30% PEG 200, 5% PEG 3350 |
